To study on the changes in saponins from callus mass by tissue culture, the callus was derived from the petiole of Korean Ginseng (Panax Ginseng C.A. Meyer) and cultivated on Murashige and Skoog´s agar medium supplemented with 2.4-dichlorophenoxyacetic acid and kinetin for 8 months.
Then, well-grown callus was analyzed for its components estimation.
The results obtained are as follows:
(1) When saponins isolated from callus mass were chromatographed on a silca gel plate, and determined by the thinchrograph TFG-10, the ratio of Rb, c to Rg(f) in saponins was 2.16 to 1 and Rb, c, d to Re, g (f) was 1 to 1.63, while in the case of saponins from the root of Panax inseng grown by soil culture, the ratio of Rb, c to Rg(f) was 1.03 to 1 and the ratio of Rb, c,d to Re, g(f) was 1 to 1.17.
(2) Sapogenins were obtained from the hydrolysates of saponins, and determined by thinchrograph TFG-10.
The ratio of panaxadiol to panaxatriol in sapogenins from callus saponins was 2.66 to 1, while he ratio of panaxadiol to panaxatriol in sapogenins from ginseng root saponins was 1.86 to 1.
From the results above mentioned, we concluded that the relative contents of sapogenins in saponins from callus mass by tissue culture were different from those in saponins from ginseng root by soil culture.
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